Katie and Ben dob mutant opercle vs WT opercle

The above images show the zebrafish opercle tagged with a bone-GFP marker in a mutant and wild-type fish. The image on the left is from the dob mutant and the image on the right is from wild-type. It seems pretty clear that there is a significant difference in the opercle size between the two. According to our collective class data, the average opercle width to length ratio for WT was 0.43 while the average ratio for the dob mutant was 0.53. This is a statistically significant difference which means that dob mutant fish do exhibit skeletal changes in comparison to WT with regards to opercle development and size.

Zebrafish Craniofacial Anatomy - Control vs. LiCl Treated Embryos - Laura

This figure shows the lateral view of the craniofacial structure of (a) an untreated zebrafish, and (b) a zebrafish treated with 10mM of LiCl. The angle of the profile of the untreated fish is 158.51°, and the angle of the profile of the treated fish is 149.93°. Treatment with LiCl results in an up-regulation of the Wnt pathway during development, thus from these results it can be inferred that an increase in the expression of Wnt results in development of a steeper craniofacial profile.

Sarah and Trent

The picture on the left is a lateral view of an untreated zebrafish embryo (our control), and the angle of its jaw is 151 degrees. The zebrafish embryo on the right has been treated with .3M LiCl. The angle of its jaw has become more steep (139 degrees) compared to the control. These results support the hypothesis that treating zebrafish embryos with LiCl causes increased activity of WNT signaling pathways during embryonic development, thus decreasing the angle of a zebrafish's craniofacial profile.

Mark and Travis sox10 control vs. sox10 fgf20 mutant

Control is on the left, fgf20 mutant is on the right. Fluorescing cells are neural crest cells. There is no distinct difference between the control and the mutant, indicating that fgf20 does not play a role in neural crest cell migration at this stage (late segmentation/early pharyngeal stage).

Alex & Jen

This is an image of a squished mutant zebrafish on a slide from lab 3. The longest fluoresced structures are the cleithrums which are membrane bones that first appear as part of the skeleton. The shorter fluoresced structures parallel to the the cleithrums are the fifth cerita branchials. This mutant has two pharyngeal teeth just like the wildtype version which are pointed inward and appear to be coming out of the fifth cerita branchials in this image.

Mark and Travis Anaphase and Telophase Movie

Grayscale movie of a cell going through anaphase and telophase.