Lab Meeting: We will have a "lab meeting" on the last day of class where each group will present their findings to the rest of the class. Each group will have 15 minutes to discuss one specific experiment as well as the results of their DNA sequence analysis.

What was the goal of the chosen experiment?

What were the results of the experiment?

Were you able to clone and identify a region or gene from the yeast genome?

If not, what step in the process was not successful and why?

If yes, what is known about this region or gene?

Lab Report: In addition to the group presentation, you will each hand in a written lab report. The format for the written report will be similar to the oral presentation, i.e. the first section will focus on describing one of the specific experiments performed in class while the second section will discuss the results of your DNA sequence analysis. The lab report should be organized as follows:

Section 1. Experiment - e.g. Restriction Enzyme Digestions

Goals: Go over the aims of the experiment. What was the overall goal of this specific experiment? Why was this experiment necessary for the ultimate goal of obtaining DNA sequence from a yeast genomic DNA fragment?

Methods: What were the experimental techniques used to accomplish the goals? What was the rationale for using these techniques?

Results: What were the results of your experiment? Be sure to go over a detailed description of your findings and show your data.

Conclusions and Discussion: Did the experiment work as planned? Was the experimental goal accomplished? If there were unexpected results, use this section to explain what have happened. How did this experiment contribute to the overall goal of the course?

Section 2. DNA Sequence Analysis

Methods: Briefly describe how the automated DNA sequencing reactions work. Be sure to discuss the Sanger dideoxy sequencing technique.

Results: How much DNA sequence were you able to obtain? What region of the yeast genome did this correspond to? Show your data.

Conclusions and Discussion: What is known about the functions or properties of the gene or genomic region you cloned?

1. Restriction Enzyme Digestions and Agarose Gel Electrophoresis of Genomic and Plasmid DNA

2. Gel purification of DNA Restriction fragments and DNA ligation

3. Bacterial Transformation and DNA Mini-Prep Analyses

4. PCR Analysis