Bio 580, Developmental Biology.  Wednesday, April 30, 1997
Overt Polarity of oocytes during the oogenic/vitellogenic process:

• Polarity of the simplest gametes of plants:  Fucus
  - pioneering work of Lionel Jaffe
  
• Polarity of animal oocytes:
  • Sea urchin
  • Amphibian
  • Bird
  • Fish
  • Insect
    - mostly Drosophila

Ionic currents and polarity:

• Currents measured in and about oocytes
  • Xenopus laevis
    - Robinson (1979) First vertebrate oocyte non-invasive measurements of ion currents.
    - voltage clamp work
  • Drosophila melanogaster
    - Overall R, Jaffe LF (1985)
    - Bohrmann J, et al.(1986)
  • Giant Silk Moths
  • Cockroaches and Termites
    - Kunkel (1991)
  
  Overheads from class
  • wire probe operation
  • wire probe callibration
  • ion probe operation and callibration
  • Periplaneta follicle
    - example of an antero-posterior ion gradient
  • Periplaneta, Zootermopsis, Eurycotis follicles
  • currents about a Blattella follicle
    - example of a dorso-ventral current
    - ions suggested by ion replacement experiments
  • Ionic currents about a cockroach follicle
    - model base on wire probe and ion replacement experiments
  • Ionic currents about a cockroach follicle
    - new model based on specific ion electrode measurements